plantiSMASH: Plant Specialized Metabolite Analysis

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Developer notice

Due to plantiSMASH development we will need to restart the service this week (17-21 March 2025), which may result in job disruption. Please resubmit your job if you encounter any issues. We apologize for any inconvenience this may cause.

To enable us to operate the plantiSMASH webserver with the resources available, users are expected to upload annotated Genbank-formatted or fasta files with gene/CDS annotations (i.e. fasta = gff3). Please first run your assembly on gene finding tools of your preference, such as AUGUSTUS or MAKER before submitting it into plantiSMASH. If you are analyzing a publicly available genome, please check the database of Precalculated Results first before submitting a job.

Search plant genome sequence for secondary metabolite biosynthesis gene clusters:

Email address (optional)

Load a file in GenBank / EMBL format (recommended) or in FASTA format

Annotation file in GFF3 format (mandatory!)

or input NCBI accession number of desired file

Additional analysis:

Gene cluster comparative analysis (ClusterBlast):

Compare to plantiSMASH predicted clusters

Compare to registered known clusters from MIBiG Database

Gene expression analysis (CoExpress):

Load a Gene Expression Omnibus (GEO) SOFT file or a properly formatted CSV file

Advanced options:

Show advanced options

CD-HIT Cutoff

: (default: 0.5)

Minimum unique domains

: (default: 2)

MAD Cutoff (CoExpress)

: (default: not set; only filter out genes with 0 MAD)

Display results for an existing job

plantiSMASH License: PlantiSMASH is licensed under the GNU Affero General Public License v3.0.